Ethan Frome By Wharton Essays (2460 words) - Ethan Frome

Ethan Frome By Wharton They say that if you give a man the necessary tools and supplies, he will build himself a trap. This trap is made unconsciously; therefore, it cannot be escaped; the solution cannot be found. The only solution that suffices is to live with this trap, sadly, for life. But is it the only solution? In Edith Wharton's romantic, yet tragic novel Ethan Frome, the need for affection causes Ethan Frome to gradually shed his taciturnity and bring his emotions to life. Early in the novel, Ethan's passiveness and lack of self-confidence, allow his wife Zeena to emasculate him, as well as make him emotionally inarticulate toward Mattie. Once Mattie Silvers enters Ethan's life, she awakens in Ethan the bitterness of his youth's lost opportunities, and a dissatisfaction with his joyless life and empty marriage. Gradually, Ethan strengthens and gathers the courage to defy Zeena and confess his love for Mattie. At the start of his journey, Ethan surrenders himself to the forces of isolation, silence, and his depleted life. Soon his desire for love, in a situation where only abject coldness exists, transforms him into an emotional and confident man. Because of his emotional weakness, Ethan loses opportunities to reveal his passion to Mattie and also acquiesces to his wife's demands, while shunning out his own needs. After suffering so long with the sickly Zeena, Ethan fears unveiling his passionate feelings to Mattie, for he is bound as a husband and tradition to Zeena. Years earlier as a younger and more hale man, Ethan felt trapped in his hometown Starkfield. Mistakenly, he marries Zeena, a gaunt, sallow nagging hag, as compensation for her nursing Ethan's sick mother. Ethan and his morose, invalid wife Zeena live in a trapped, unspoken resentment on Ethan's isolated and failing farm. Driven by a perverted need for attention, Zeena claims to have numerous ailments and employs her destitute cousin to help with the chores. Over the course of the years, Ethan, lonely and miserable, finds himself falling in love with Mattie, drawn to her youthful, animated energy. As he walks through town one night, he stops by the church to watch Mattie dance. As he marvels at the young girl's beauty he thinks, "But hitherto the emotion had remained in him as silent ache, veiling with sadness the beauty that evoked it. He did not even know whether any one else in the world felt as he did, or whether any one else in the world felt as he did, or whether he was the sole victim of this mournful privilege"(17). Ethan lives in his own world of silence, where he replaces his scarcity of words with imaginations and fantasies. For years Ethan and his wife live in silence and seclusion. Ultimately, the total lack of communication between the silent couple significantly contributes to their miserable marriage as well as Ethan's inability to act out his emotions. Ethan, accustomed to his silent relationship with his wife, flutters when he meets Mattie and loses complete faith in himself. Fear and doubt overwhelms because love is a new concept to him; a journey he scarcely ventures. Every time Ethan tries to converse with his wife, all he ever hears are her complaints and demands. Thus, he would rather not communicate with her at all. Also, since Zeena looks down on Ethan, he feels that he also must not be worthy enough for Mattie. He believes Zeena's criticisms and they taunt him when he attempts to court Mattie. Ethan further displays uncertainty and shyness by restraining himself from kissing Mattie. The morning after Ethan escorted Mattie home, Ethan's thoughts turn back to last night. As the memory of Mattie's warm shoulder comes back to him, he regrets his failure to kiss her when he had the chance. Ethan, frustrated, asks himself, "Why had he not kissed her when he held her there? . . . a few minutes earlier, when they had stood alone outside the house, he would not have dared to think of kissing her" (29). As a cold, isolated, and grim figure, Zeena embodies her surrounding. She creates a loveless, desolate home for Ethan where he never learns to express his love and affection. When the moment to manifest his passion to Mattie in the form of the kiss arrives, he becomes nervous, and shies out like a scared lamb. Even more, Ethan lacks the confidence and courage to stand up for himself against Zeena, who manipulates Ethan and uses her frail health to justify her bitter personality. When Zeena

Changing to War essays

Changing to War essays Three of the major causes of the Civil War deal mainly with the idea that the Souths Break Up was a direct result from the lack of consideration that the North had. Each cause was created by a sort of tension between the two. Each had their opposite ideas of how the U.S. should be ran, along with the way of living. Mainly tensions were brought up and about from slavery decisions such as the Dred Scott Decision, the presidency of the time, since it needed to be an asset to the time for both the North and South, and the Territory of which the peoples needed to be decided on whether they were to be pro or anti slavery, mainly dealing with the Kansas Nebraska Act. Each created tensions that grew as time went along, which also began to raise emotions. In 1854 Congress passed the Kansas-Nebraska Act. The Kansas-Nebraska Act was a U.S. law authorizing the creation of Kansas and Nebraska, west of the states of Missouri and Iowa and divided by the 40th parallel. It repealed a provision of the Missouri Compromise that had prohibited slavery in the territories north of 36Â ° 30', and stated that the people of the territories should decide for themselves the validity of slaveholding. This was known as popular sovereignty. Those did not gain anything from this law and other opponents of slavery responded by forming the Republican Party. Popular sovereignty soon degenerated into a civil war in Kansas, known as the Border War, or Bleeding Kansas, as Southerners and Northerners battled over the status of slavery. The Kansas-Nebraska Act was sponsored by the Democratic senator Stephen A. Douglas of Illinois. By opening up what had been Native American country to white settlement, Douglas and other northern leaders hoped to facilitate construction of a transcontinental railroad through their states rather than through the southern part of the country. The removal of the restriction on the expansion of slavery ensured s ...

Endogenous Growth Theory Essay Example | Topics and Well Written Essays - 750 words

Endogenous Growth Theory - Essay Example The paper focuses on endogenous growth and utilises two major approaches to deal with the issue. These are namely the convergence controversy approach and the state of market competition (Romer). Romer’s contention is to fill out gaps in existing endogenous theory to make it more accountable for growth patterns in developing and developed countries. Historically the neo-classical model has attributed economic growth to technology. Another basic assumption is that technology is freely available to all countries in the world since a perfect competition market exists (Rebelo). Romer uses the Cobb-Douglas production and cross-country regression models in order to highlight that endogenous factors can better explain such growth than exogenous models. He attributes growth to investments in human capital, innovation and knowledge whose spill over effects tend to augment the economy as a whole . The Philippines has been compared to the United States in order to bring out the savings rates that would be required by both nations to possess an equal level of economic growth. Romer argues that if the Philippines and the United States possessed the same level of technology, then their differing growth rates could be attributed to differences in labour productivity alone. It has been estimated that the share of investment in the United States is at least twice as large as it would have to be in the Philippines for a similar rate of growth. The lack of convergence between the growth rates for poorer countries and the more rich countries tends to indicate that the differences may be attributed to more than just technology. The rate of and amount of investment tends to differ between the North and the South. Using the neo-classical model, it would be hard to explain why the model attributes low investment in the North while that is not the case. The Summer-Heston model has been used in this regard to look into investments into human resources and capital to bring out the differences (Barro and Sala-i-Martin). Romer concludes this section by delineating that the only difference between developing and developed nations may not just be the availability of data that is blamed by neo-classical economists for a loosely fitting model. In the second section, Romer argues that aggregate level models had been missing in order to exp lain growth throughout the fifties, sixties and the seventies. He also expounds that certain assumptions have always been assumed as such but have not been explored to see their effects on growth models. It is generally assumed that there are many firms in an economy but it may be that these are concentrated to favour a monopolistic market structure. Scientific discoveries are not accessible to all entities operating in an economy since information is required to turn the scientific discovery into useful output. The shortage of information from one national economy to another (such as through trade secretes) signifies that scientific discoveries are not available to all and sundry. Another issue is the replication of physical activities which is not possible since all involved factors cannot be scaled up similarly all the time to receive an equally scaled up output. Technological developments are taken as having derived from factors external to the control of individuals. However, t he application of human resources and attention is typically how technological progress is derived. Hence, assuming that technological progress is an exogenous factor is highly misleading (Sachs and Warner). Additionally, economic entities with the power to create new information and knowledge often possess the power to manipulate the information and knowledge into monopolistic systems. This would indicate that the owner of the information a

Capital Funding Essay Example | Topics and Well Written Essays - 3500 words

Capital Funding - Essay Example Contrary to this as per the advocates of capital funding, it causes encouragement of the development of technologies, managerial expertise, and integration with the world economy, exports and higher growth. Since it is a very much controversial issue, my primary objective will be exploring the all the aspect of capital funding in new business and private sector in developing countries and also calculating possibility and to answer the hypothesis that effect of the various policies of capital funding on the development of new business and private practice in developing countries. Since this research problem needs an in depth study and it give rise to some insights to the magnitude of the capital funding I'll formulate such research questions which may help me exploring the reality of the problem. The questions will be, Since it is highly necessary to gather accurate information for giving an ample scope to my research problem, I will approach autonomous and governmental bodies like Department for international development, office of foreign common wealth offices and HM treasury. Collecting data from such bodies will help me to draw a comprehensible data related to the effect of policies in the development of new business and private practice in developing countries. ... The governments of respective countries has implemented a number of policies related to this .But The practice of imparting and accepting the fund always invites criticism as the opponents draw attention regarding imperfections, adverse results such as capital intensity of such funds, inappropriate technology ,the possible adverse on income distribution etc. Contrary to this as per the advocates of capital funding, it causes encouragement of the development of technologies, managerial expertise, and integration with the world economy, exports and higher growth. Since it is a very much controversial issue, my primary objective will be exploring the all the aspect of capital funding in new business and private sector in developing countries and also calculating possibility and to answer the hypothesis that effect of the various policies of capital funding on the development of new business and private practice in developing countries. Since this research problem needs an in depth study and it give rise to some insights to the magnitude of the capital funding I'll formulate such research questions which may help me exploring the reality of the problem. The questions will be, 1. What is the magnitude of the effectiveness of the policies in developing countries, particularly in their economic growth 2. Whether it effect positively or negatively on the development of new business and private practice 2 3. What are aims of the particular government regarding the implementation of the respective policies 4. Which are major countries who undertaken to impart funds to developing countries and whether there is particular motive

Business Ethics and Deontology Essay Example | Topics and Well Written Essays - 1750 words

Business Ethics and Deontology - Essay Example Moreover, the field of ethics in the business module observes ethical controversies with regard to various social responsibilities within the business process and also includes the ethical conditions of the professional entities, the fundamental rights of the employees, employment discrimination and positive actions among other concerns (Scribd Inc., 2012). The objective of this paper is to identify three major ethical issues that were raised in the WorldCom case in its various business processes and to evaluate those issues using the deontological ethics guidelines. Moreover, the ethical issues faced by WorldCom will also be critically assessed through implementing Kant’s Categorical Imperative model of business ethics. Three Major Ethical Issues of WorldCom An ethical controversy significantly affected WorldCom during its business operations and most importantly upon its sustainability in the business market. The organization had faced financial distress during the scenario of US bankruptcy in the year 2001. However, WorldCom emerged from bankruptcy fortification after reporting its accounting irregularities of USD 11 billion. The accounting irregularities in WorldCom had resulted in its being prosecuted against security charges of many of the organization’s senior executives (Scharff, 2005). ...Corporate Acquisition Strategy of WorldCom The core business strategy of WorldCom was highly focused to develop the organizational growth through an effective acquisition strategy which was quite complex and a lengthy process of operation. Mergers and acquisitions process in an organization is a delicate process to efficiently execute and can cause various problems in order to effectively overcome the concerns (Cernusca, n.d.). WorldCom had performed 65 acquisitions worldwide through its effective business strategies and was regarded as one of the major business organizations in the telecommunication industry. WorldCom had spent almost USD 60 billion bet ween the years 1991 to 1997 in its acquisition processes and accumulated a debt amount of more than USD 40 billion (Moberg & Romar, 2012). The acquisition strategy of the organization significantly focused on acquiring larger firms across the different regions of the world. The acquired organizations facilitated WorldCom to achieve larger market share along with strengthening their core competencies, however, it was observed that WorldCom failed to generate desired profit from its acquired organizations. The major ethical issues were raised due to various types of accounting scandals as well as certain unethical practices in the accounting department of the organization (Davis, Miksiewicz, Nitta, Rothenberger, & Scalera, 2011).

Sterilization And Aseptic Technique Biology Essay

Sterilization And Aseptic Technique Biology Essay This experiment was done to learn proper way of using aseptic technique and sterilization by isolating pure culture of bacterial. Afterwards, the bacterial cells in a sample and their optical density were determined. First of all, various sterilization methods were introduced. Sterilization is important in a sense that it ensures there is absolutely no contamination in the glassware or apparatus used in the lab. Different sterilization methods are used for different materials. One of them is autoclaving. Autoclaving machine uses high-pressure steam to sterilize and therefore, heat resistant plastics, glass or solutions can be sterilized by autoclaving. As the temperature of the steam is above 100 oC, the organisms cannot survive. Second sterilization method is radiation. As heat sensitive plastics does not have resistance to heat, autoclaving cannot be used and these are often sterilized by using radiation such as UV, gamma-ray or X-ray. The last method is filter sterilization. Some solutions are heat labile, and to sterilize these kind of solutions, filter sterilization can be used. This technique uses the fact that microorganism is around 5micrometer by 1micrometer, and if the filter has a smal ler diameter, microorganisms cannot pass through the filter.(1) In part B, aseptic technique is learned. This technique prevents any kind of contamination while handling the glassware or transferring. To be more specific, it prevents any contaminant to be introduced in the area of interest. The first step of this technique involves wiping the lab bench with 70% ethanol, which would kill most microorganisms. Then, Bunsen burner is turned on, and the movement of the air goes upwards. Therefore, it minimizes the chance of microorganisms landing on the media of interest. In addition, briefly heating glass tube mouths and minimizing the time of opening lids minimizes contamination.(1) Using aseptic technique, streaking technique was used to isolate single colonies. To do this, a pure culture of the target microorganism is taken. Then, with an inocular loop, which is flamed with Bunsen burner until red hot, it cooled down. Afterwards, take a bit of pure culture with the loop and streak lines in the medium. The streaking lines should not cross each other to avoid too much diluting. After streaking, colonies are grown. To count the number of cells, viable cell count method is used. Viable count is only useable with singles colonies and not bacterial lawns. Therefore, in order for cell to have single colonies, appropriate dilution of the bacteria is necessary. The dilution helps for spreading of the cells on the agar. For this, serial dilution, which was introduced last project can be useful. Then, the number of viable cells can be obtained by counting the number of colonies that have developed multiplied by the respective dilution factor. (2) Material and Methods: All procedures are performed according to the BIOL 368 lab manual (Concordia Biology Department 2013) except for the following modifications: for the contamination part, we used shoe, finger, E. coli, and E. coli with 70% ethanol. Results: Colony isolation by streaking First of all, the color of the bacteria in all the plates are thick beige colored. In streak 1, extremely small and many colonies were observed. The size of the colonies were very small, they were circular, opaque and smooth. There are 123 colonies. Streak 2 shows chain of bacterial formation, but the number of the colonies is decreased from streak 1. The number of colonies were 60. They were larger than the colonies in streak 1, opaque, circular and smooth as well. In streak 3, single colonies are observed. None of them was huge, but they were larger than the colonies from streak 2. They were opaque, circular and smooth as well. About 9 colonies were observed. In the 4th streak, no single colony was observed. As a result, single colonies of a pure E. coli strain was successfully isolated. Viable count Table 1. Raw data of viable count of my group. Dilution 10-4 10-5 10-6 Number of colony Too many Too many 252 Viable count (cfu/ml) 2.52 x 109 Sample calculation: Viable count at 10-6 dilution: Since the plate, -6, has 252 colonies which is in the range of 100-300, I picked the plate to calculate cfu/ml. 252 x 10/10-6 =2.52 x 102 x 107 =2.52 x 109 cfu/ml Table 2. Raw data for viable count for all section Dilutions Colony count Group 1 Group 2 Group 3 Group 4 Group 5 Group 6 Group 7 Group 8 Group 9 Group 10 Section 1 10-4 lawn lawn lawn lawn lawn lawn lawn à £Ã¢â€š ¬Ã¢â€š ¬ lawn lawn 10-5 360 lawn 1848 lawn 1028 2168 696 à £Ã¢â€š ¬Ã¢â€š ¬ 3040 1646 10-6 78 287 441 270 234 347 363 à £Ã¢â€š ¬Ã¢â€š ¬ 300 306 Section 2 10-4 920 >300 Lawn Lawn Lawn >300 >300 Lawn Lawn Lawn 10-5 249 >300 590 >300 Lawn >300 >300 406 >300 189 10-6 23 231 189 269 384 222 265 154 180 108 Section 3 10-4 too many >1000 too many too many too many too many too many too many too many too many 10-5 too many >1000 too many too many too many too many too many too many 544 too many 10-6 194 420 258 252 295 217 240 197 79 224 Table 3. Viable count for all section data (for 10-6 dilution) Section Group Number of colonies Cfu/ml 01 1 78 7.8.E+08 2 287 2.87.E+09 3 441 4.41 E+09 4 270 2.70 E+09 5 234 2.34.E+09 6 347 3.47E+09 7 363 3.63E+09 8 9 300 3.00E+09 10 306 3.06E+09 Max 441 4.41E+09 Min 78 7.8 E+08 Average 291.8 2.92 E+09 Standard Dev. 94.40 2.67E+08 02 1 23 2.3.E+08 2 231 2.31.E+09 3 189 1.89.E+09 4 269 2.69.E+09 5 384 3.84.E+09 6 222 2.22.E+09 7 265 2.65.E+09 8 154 1.54.E+09 9 180 1.80.E+09 10 108 1.08.E+09 Max 384 3.84.E+09 Min 23 2.3.E+08 Average 202.5 2.02.E+09 Standard dev. 93.09 9.31E+08 03 1 194 1.94.E+09 2 420 4.20.E+09 3 258 2.58.E+09 4 252 2.52.E+09 5 295 2.95.E+09 6 217 2.17.E+09 7 240 2.40.E+09 8 197 1.97.E+09 9 79 7.9.E+08 10 224 2.24.E+09 Max 420 4.20.E+09 Min 79 7.9.E+08 Average 237.6 2.38.E+09 Standard Dev. 81.55 8.16.E+08 Max 441 4.41.E+09 Min 23 2.3.E+08 Average 242.3 2.42.E+09 Standard Deviation 96.75 1.15E+09 Sample calculation for STD DEV. (section 1):= 94.40 Bacterial cell count by optical density Table 4. Cell density for My Group: OD600 of diluted cultures (Au) E. Coli Count of diluted Culture (cells/ml) Original Culture (cells/ml) Me 0.427 2.18108 2.18109 Partner 0.436 2.14108 2.14109 E. Coli Count of diluted Culture: 0.202 x (5x 108) = 1.01108 Original Culture: 1.01108 x 10 = 1.01109 Table 5. Raw OD600 values for all sections (unit: Au) Group Section 1 (1) Section 1 (2) Section 2 (1) Section 2(2) Section 3(1) Section 3 (2) 1 0.389 0.383 0.359 0.371 0.358 0.365 2 0.368 0.369 0.247 0.447 0.345 0.408 3 0.364 0.343 0.344 0.360 0.323 0.335 4 0.374 0.374 0.390 0.338 0.427 0.436 5 0.415 0.430 0.386 0.368 0.320 0.247 6 0.359 0.357 0.302 0.350 0.379 0.352 7 0.347 0.372 0.354 0.369 0.391 0.364 8 0.362 0.361 0.358 0.328 9 0.370 0.35 1.018 0.350 0.341 10 0.737 0.367 0.368 0.353 0.413 0.322 (>1.96 therefore outlier) Table 6. Diluted Cell Density for all sections (unit: cells/ml) Group Section 1 (1) Section 1 (2) Section 2 (1) Section 2(2) Section 3(1) Section 3 (2) 1 1.95E+08 1.92E+08 1.80E+08 1.51E+08 1.79E+08 1.90E+08 2 1.84E+08 1.85E+08 1.86E+08 1.75E+08 1.83E+08 1.76E+08 3 1.82E+08 1.72E+08 1.24E+08 1.77E+08 1.73E+08 1.96E+08 4 1.87E+08 1.87E+08 2.24E+08 1.85E+08 2.04E+08 1.82E+08 5 2.08E+08 2.15E+08 1.72E+08 1.81E+08 1.62E+08 1.79E+08 6 1.80E+08 1.79E+08 1.80E+08 1.81E+08 1.68E+08 1.64E+08 7 1.74E+08 1.86E+08 1.95E+08 1.75E+08 2.14E+08 1.75E+08 8 1.69E+08 5.09E+08 2.18E+08 1.71E+08 9 1.85E+08 1.93E+08 1.84E+08 1.60E+08 2.07E+08 10 1.87E+08 1.84E+08 1.84E+08 1.77E+08 1.24E+08 1.61E+08 Average 1.87E+08 1.95E+08 1.79E+08 Min 1.72E+08 1.24E+08 1.24E+08 Max 2.15E+08 5.09E+08 2.18E+08 Standard Deviation 1.05E+07 7.43E+07 2.13E+07 Sample calculation: Section 1 group1 student 1: Cell Density = 0.389 x (5x 10^8) = 1.95 x107 Section 1 Average: Average = ((1.95+1.84+1.82+1.87+2.08+1.80+1.74+1.85+1.87+1.92+1.85+1.72+1.87+2.15+1.79+1.86+1.84) x 108)/ 17 = 1.87 x108 Table 7. Diluted Cell Density for all sections, class analysis Class (cells/ml) Average 1.87E+08 Minimum 1.24E+08 Maximum 5.09E+08 Standard Deviation 4.66E+07 Part III. The ubiquity of microorganisms Table 8. The ubiquity of microorganisms Place Observation on TSA plate Observation on Malt Shoe Irregular orange, red, beige opaque Some are large, some are small Some are smooth some are cracked 10colonies None Dirty finger White and yellow all small colonies (4) opaque None E.coli Few circular, smooth, flat, beige colonies (lawn) None E.coli with 70% ethanol None None Discussion: The objective of the experiment is to learn aseptic technique, sterilization, and streaking. Part A involved isolating single colonies by streaking, part B involved viable cell count, part C involved bacterial cell count by optical density and lastly part D involved ubiquity of microorganisms. In part A, a pure E. Coli sample was used to form single colonies by streaking. Four streaks were done in different parts of the plate. As described in the results, 1st streak results in forming smallest and very crowded colonies (123 colonies). The space between the colonies were either very small or even adjacent to each other. The 2nd streak forms a larger and less crowded or less population of colonies (60 colonies). Colonies were found to be further apart from streak 1, but they were adjacent to other colonies, so single colonies were not observed. In the 3rd streak finally, isolated single colonies were observed. This is due to the dilution. As in the streak 1, we have least diluted E. Coli pure sample. Therefore, streak 1 has the biggest cell density, where more colonies would grow. In the streak 2, we streak through the streak 1 once, and so, it is diluted. Then, in the third streak as well, it is even more diluted. This is why we have lesser and lesser colonies in the 2nd an d 3rd colonies. Colonies all seem to have same opaque shape with beige color, but they differ in sizes. 1st streak ones have the smallest and 3rd streak ones have the largest. This is due to the fact that as the number of the colonies are bigger and crowded, there are less space to grow, so it tends to be smaller where as in 3rd streak, isolated colonies have more space where they can grow bigger. In part B, viable count was used to estimate the number of bacterial cells in the sample. Firstly, we prepared 4-fold, 5-fold and 6-fold diluted solutions of E. Coli and they were incubated at 37 degrees Celsius. As we can see in the table 2, 4-fold dilution and 5-folded dilution are too concentrated that bacterial lawn is observed where we cannot apply viable count: they have small viable count to work with and would result in high uncertainty (1). 6-folded dilution appears to be fine to apply viable count and therefore, we used 6-folded dilution to analyze. Looking at the all section data, most of them have the viable counts ranged between 30 and 300. In addition, the average viable count of our section is very close to the class average: 2.38 x 109 to 2.42 x 109 cfu/ml. Also, my group value is very close to the class average as well: 2.52 x 109 cfu/ml. This means that our result is pretty accurate compared to the class result. Speaking of the minimum and the maximum value, there i s a high chance that the errors come from these as these values are furthest from the average. Section 1 has the maximum value which is 4.41 x 109 cfu/ml and section 2 has the minimum value which is 2.3 x 108 cfu/ml. The minimum value seem to be okay but maximum value seem to lie over 300 colonies, and therefore, the biggest error comes from that value. However, none of these biggest error comes from our section, and therefore, we can say that our section value has the least error. Part C was done to take cell density by optical density. CAG12033 was taken and was diluted with LB broth. When analyzing, the group 9 student2s value was 1.018, which appeared to be as an outlier. Grubbs test was done, and it was eventually an outlier, so we excluded it from further analysis. Speaking of the cell density, as there are more and more of cell in the solution, the density increases. As well, the result shows that as absorbance increases, the cell density increases. Comparing the class average to our section average, it is fairly close: 1.79x 108 and 1.87 x 108cells/ml. However, we have the class minimum value which is 1.24 x 108 cells/ml so we have one of the largest errors. But this is not very far from the average value, which is 1.87 x 108 cells/ml it is not the biggest error. The class maximum value however is very far from the class average value: 5.09108 cells/ml. This value is in the section 2 data. Section 1 has the best result over the class with closest averag e value to the class average having no minimum nor maximum values; 1.87 x 108cells/ml which is the same as class average. Comparing my cell density value to the section value, I had 2.18x109cells/ml, whereas the class average was 1.87 x 108 cells/ml. I have a fairly close value and it can be considered that CAG12033 is diluted fairly correctly. Now comparing viable count method to the cell density measured by spectrophotometer, they can be considered the same. The class average value for the cell density was1.87 x 108 cells/ml and the class average result for the viable count method was 2.42 x 109 cfu/ml. They can be considered the same with the following reasons. First of all, for the optical density method, there is an assumption that there are 5 x 108 cells/ml when the absorbance is 1 Au. This is an assumption and is not an accurate value. Secondly, there are experimental errors such as when diluting, the dilution was not done perfectly, where the error would increase as serial dilution was done in viable count part. As a result, factor of 10 difference is quiet big, but within these assumptions and errors, they can be considered as similar. Part D was done to see what contamination looks like and how it is. TSA and malt medium were used to contaminate. Different samples were taken with a sterilized rod and were streaked different parts of the plates on both TSA and malt. They were then incubated at 37 degrees Celsius if it is from internal body or incubated at 30 degrees otherwise. TSA is usually considered the best under neutral to slightly basic conditions and required high N for bacteria to grow. On the other hand, malt is best under acidic condition and high in C and N. Malt is best for fungi. First of all, the shoe was rubbed, and streaked on both TSA and Malt plates. A week later, all different kinds of bacteria were grown. Various colored and various sizes were observed: orange, red and beige. Some were really huge and flat, some were small, opaque and smooth. 10 colonies were observed. On malt, nothing grew. Due to the fact that nothing grew on malt, the colonies have to be bacteria. Another possibilities is tha t malt plate was put in the 37 degrees Celsius which is inappropriate. In quarter of the plate, dirty finger was used to contaminate. 4 colonies of white and yellow were observed. They were all opaque. Nothing grew on malt. In another part of the plate, we put E. Coli sample. Circular, smooth, flat colonies were observed. There were a lot of colonies (bacterial lawn) grown. Again nothing grew on malt. Lastly, we put E. coli with 70% ethanol. Absolutely nothing grew on both malt and TSA. Overall, nothing grew on malt. It is maybe because there was no fungi, or the plates were incubated in the wrong temperature (37 degrees Celsius instead of 30 degrees Celsius). Also, we can say that 70% ethanol kills most of the bacteria or at least enough to prevent them to grow.

Iran- Contra Scandal :: essays research papers

Over the centuries ancient Roman society has played a significant role in the creation of a common culture like our own. The material remains from ancient Rome have preserved valuable evidence for the status and accomplishments of the Roman people. Because so many aspects of ancient Roman civilization are respected and followed in society today, such as Roman art, Roman roads, and Roman law, it is important to understand the similarities and differences that ally within the two cultures. One aspect of Roman culture that I found interesting to focus on is the tradition of marriage. A traditional Roman marriage is completely different than a traditional marriage in our culture today, essentially because of the significant change in the role of women over time. Because Roman women had hardly any rights, their role in marriage was extremely limited. Women in ancient Rome barely had any rights by law, so they had very few opportunities to live independently. Roman women were expected to marry at a young age, usually when they reached their teen years or early twenties. A Roman woman's subordination in marriage began even before she became engaged, as it was tradition for a woman's father, or legal guardian to arrange the marriage of their daughters. In her work on Roman Marriage, Susan Treggiari sites a preserved document of a Roman mother explaining the rules of engagement to her daughter: "When you become engaged to someone, your father, will inform you of the fact. An engagement should come on a young girl as a surprise, pleasant or unpleasant, as the case may be. It is hardly a matter that she could be allowed to arrange for herself" (Treggiari, 125). The legal independence of women was also constrained by the institution of guardianship. If a woman outlived her father, it was required by law in Rome to ha ve a guardian, or tutor. The legal sources about the reasons women needed guardians are specific, as they describe women as lacking judgment, were easily victimized, basically because they claimed women were fools. Roman women were expected to be married, primarily because it was almost impossible for them to live independently, but also because single women, or maidens, were just not accepted in society. Most single women though were of the lower class, and had little opportunity to obtain a better life. "Free-born women continued to scratch our a living as laundresses, weavers, butchers, and fish-sellers, or in one of the occupations that are recorded on inscriptions at Pompeii: bean-dealer, nail-seller, brick-maker, even stonecutter.